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Publication Metadata only Spectral insights: Navigating the frontiers of biomedical and microbiological exploration with Raman spectroscopy(ELSEVIER SCIENCE SA, 2024) Allakhverdiev, Elvin S.; Kossalbayev, Bekzhan D.; Sadvakasova, Asemgul K.; Bauenova, Meruyert O.; Belkozhayev, Ayaz M.; Rodnenkov, Oleg, V; Martynyuk, Tamila, V; Maksimov, Georgy, V; Allakhverdiev, Suleyman I.; National Medical Research Center of Cardiology; Lomonosov Moscow State University; Akhmet Yassawi International Kazakh-Turkish University; Chinese Academy of Sciences; Tianjin Institute of Industrial Biotechnology, CAS; Al-Farabi Kazakh National University; Satbayev University; Satbayev University; Aitkhozhin Institute of Molecular Biology & Biochemistry; Timiryazev Institute of Plant Physiology; Russian Academy of Sciences; Bahcesehir UniversityRaman spectroscopy (RS), a powerful analytical technique, has gained increasing recognition and utility in the fields of biomedical and biological research. Raman spectroscopic analyses find extensive application in the field of medicine and are employed for intricate research endeavors and diagnostic purposes. Consequently, it enjoys broad utilization within the realm of biological research, facilitating the identification of cellular classifications, metabolite profiling within the cellular milieu, and the assessment of pigment constituents within microalgae. This article also explores the multifaceted role of RS in these domains, highlighting its distinct advantages, acknowledging its limitations, and proposing strategies for enhancement.Publication Metadata only Biotechnological production of hydrogen: Design features of photobioreactors and improvement of conditions for cultivating cyanobacteria(PERGAMON-ELSEVIER SCIENCE LTD, 2024) Kossalbayev, Bekzhan D.; Yilmaz, Girayhan; Sadvakasova, Asemgul K.; Zayadan, Bolatkhan K.; Belkozhayev, Ayaz M.; Kamshybayeva, Gulzhanay K.; Sainova, Gaukhar A.; Bozieva, Ayshat M.; Alharby, Hesham F.; Tomo, Tatsuya; Allakhverdiev, Suleyman I.; Satbayev University; Satbayev University; Al-Farabi Kazakh National University; Bahcesehir University; Aitkhozhin Institute of Molecular Biology & Biochemistry; Akhmet Yassawi International Kazakh-Turkish University; Timiryazev Institute of Plant Physiology; Russian Academy of Sciences; King Abdulaziz University; Tokyo University of ScienceOver the last five decades, solar-based hydrogen (H2) production has been intensively studied. Specifically, the study of biophotolysis by cyanobacteria has received great attention to produce H2, and promising research approaches have been established. To date, numerous photobioreactors (PBRs) have been built to collect cyanobacterial biomass and generate bioenergy. Additionally, different PBR parameters were adjusted to increase the product yield. PBR development holds great potential not only for cell biomass but also for biological H2 production. This review aimed to examine the mechanisms involved in H2 production by cyanobacteria, explore the factors influencing the process, and describe five distinct PBRs known for their high H2 production. This article examines the pros and cons of the most efficient PBRs for H2 production and offers insights into strategies for (c) 2023 Hydrogen Energy Publications LLC. Published by Elsevier Ltd. All rights reserved.Publication Metadata only Prospects of cyanobacterial pigment production: Biotechnological potential and optimization strategies(Elsevier B.V., 2022) Sandybayeva, Sandugash K.; Kossalbayev, Bekzhan D.; Zayadan, Bolatkhan K.; Sadvakasova, Asemgul K.; Bolatkhan, K.; Zadneprovskaya, Elena V.; Kakimova, Ardak B.; Alwasel, Salah Hamad; Leong, Yoong Kit; Allakhverdiev, Suleyman I.; Sandybayeva, Sandugash K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan; Zayadan, Bolatkhan K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Bolatkhan, K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Zadneprovskaya, Elena V., Controlled Photobiosynthesis Laboratory, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation; Kakimova, Ardak B., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Alwasel, Salah Hamad, College of Sciences, Riyadh, Saudi Arabia; Leong, Yoong Kit, Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan; Allakhverdiev, Suleyman I., Controlled Photobiosynthesis Laboratory, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation, Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, TurkeyIncreasing awareness of the harmful effects of synthetic colorants has led consumers to favor the use of natural alternatives such as plant or microbial pigments in food and cosmetics. Cyanobacteria are a rich source of many natural biopigments that are of high commercial value. In the market, bio-based pigments are usually sold as extracts to reduce purification costs. Various cell disruption methods are used for pigment extraction, such as sonication, homogenization, high pressure, supercritical CO2 extraction, enzymatic extraction, as well as other promising novel extraction methods that make the production of cyanobacterial pigments economically viable. In addition, a continuous cultivation system is considered the most suitable cultivation mode for large-scale biomass production. However, a major limitation in the large-scale production of cyanobacterial pigments is the installation and operation costs. Thus, basic and applied research is still needed to overcome such limitations and enable cyanobacteria to enter the global market. This review focuses on various cyanobacterial pigments, their applications, and current biotechnological approaches to increase the production of biopigments for their potential use in the pharmaceutical, food, and cosmetic industries. The current state of production technologies based on either open pond systems or closed photobioreactors was compared. The potential of scientific and technological advances to increase yield and reduce production costs of cyanobacteria biomass-based pigments was also discussed. © 2022 Elsevier B.V., All rights reserved.Publication Metadata only Synthetic algocyanobacterial consortium as an alternative to chemical fertilizers(Academic Press Inc., 2023) Sadvakasova, Asemgul K.; Bauenova, Meruyert O.; Kossalbayev, Bekzhan D.; Zayadan, Bolatkhan K.; Huang, Zhiyong; Wang, Jingjing; Balouch, Huma; Alharby, Hesham Fasial; Chang, Jo-Shu; Allakhverdiev, Suleyman I.; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Bauenova, Meruyert O., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan; Zayadan, Bolatkhan K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Huang, Zhiyong, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Wang, Jingjing, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Balouch, Huma, Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Alharby, Hesham Fasial, Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Chang, Jo-Shu, Department of Chemical and Materials Engineering, Tunghai University, Taichung, Taiwan, Research Center for Smart Sustainable Circular Economy, Tunghai University, Taichung, Taiwan, National Cheng Kung University, Tainan, Taiwan, Department of Chemical Engineering and Materials Science, Yuan Ze University, Taoyuan, Taiwan; Allakhverdiev, Suleyman I., Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation, Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, TurkeyThe use of unregulated pesticides and chemical fertilizers can have detrimental effects on biodiversity and human health. This problem is exacerbated by the growing demand for agricultural products. To address these global challenges and promote food and biological security, a new form of agriculture is needed that aligns with the principles of sustainable development and the circular economy. This entails developing the biotechnology market and maximizing the use of renewable and eco-friendly resources, including organic fertilizers and biofertilizers. Phototrophic microorganisms capable of oxygenic photosynthesis and assimilation of molecular nitrogen play a crucial role in soil microbiota, interacting with diverse microflora. This suggests the potential for creating artificial consortia based on them. Microbial consortia offer advantages over individual organisms as they can perform complex functions and adapt to variable conditions, making them a frontier in synthetic biology. Multifunctional consortia overcome the limitations of monocultures and produce biological products with a wide range of enzymatic activities. Biofertilizers based on such consortia present a viable alternative to chemical fertilizers, addressing the issues associated with their usage. The described capabilities of phototrophic and heterotrophic microbial consortia enable effective and environmentally safe restoration and preservation of soil properties, fertility of disturbed lands, and promotion of plant growth. Hence, the utilization of algo-cyano-bacterial consortia biomass can serve as a sustainable and practical substitute for chemical fertilizers, pesticides, and growth promoters. Furthermore, employing these bio-based organisms is a significant stride towards enhancing agricultural productivity, which is an essential requirement to meet the escalating food demands of the growing global population. Utilizing domestic and livestock wastewater, as well as CO2 flue gases, for cultivating this consortium not only helps reduce agricultural waste but also enables the creation of a novel bioproduct within a closed production cycle. © 2023 Elsevier B.V., All rights reserved.Publication Metadata only Biotechnological production of hydrogen: Design features of photobioreactors and improvement of conditions for cultivating cyanobacteria(Elsevier Ltd, 2024) Kossalbayev, Bekzhan D.; Yilmaz, Girayhan; Sadvakasova, Asemgul K.; Zayadan, Bolatkhan K.; Belkozhayev, Ayaz; Kamshybayeva, Gulzhanay K.; Sainova, Gaukhar Askerovna; Bozieva, Ayshat M.; Alharby, Hesham Fasial; Tomo, Tatsuya; Kossalbayev, Bekzhan D., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Yilmaz, Girayhan, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, Turkey; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Zayadan, Bolatkhan K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Belkozhayev, Ayaz, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan, M. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Kazakhstan; Kamshybayeva, Gulzhanay K., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Sainova, Gaukhar Askerovna, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan; Bozieva, Ayshat M., Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation; Alharby, Hesham Fasial, Department of Biological Sciences, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia; Tomo, Tatsuya, Department of Physics, Tokyo University of Science, Tokyo, JapanOver the last five decades, solar-based hydrogen (H2) production has been intensively studied. Specifically, the study of biophotolysis by cyanobacteria has received great attention to produce H2, and promising research approaches have been established. To date, numerous photobioreactors (PBRs) have been built to collect cyanobacterial biomass and generate bioenergy. Additionally, different PBR parameters were adjusted to increase the product yield. PBR development holds great potential not only for cell biomass but also for biological H2 production. This review aimed to examine the mechanisms involved in H2 production by cyanobacteria, explore the factors influencing the process, and describe five distinct PBRs known for their high H2 production. This article examines the pros and cons of the most efficient PBRs for H2 production and offers insights into strategies for increasing their productivity. © 2023 Elsevier B.V., All rights reserved.Publication Metadata only Combating Phytopathogens by Integration of Metagenomics and Phototrophic Biotechnologies: Toward Sustainable Agricultural Practices(Taylor and Francis Ltd., 2025) Sadvakasova, Asemgul K.; Kossalbayev, Bekzhan D.; Zaletova, Dilnaz E.; Bauenova, Meruyert O.; Huang, Zhiyong; Zharmukhamedov, Sergei K.; Shabala, Sergey Nikolayevich; Allakhverdiev, Suleyman I.; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan; Zaletova, Dilnaz E., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Bauenova, Meruyert O., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Huang, Zhiyong, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Zharmukhamedov, Sergei K., Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation; Shabala, Sergey Nikolayevich, School of Biological Sciences, The University of Western Australia, Perth, Australia; Allakhverdiev, Suleyman I., Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, TurkeyRising global food demand amid climate change presents unprecedented challenges for modern agriculture. The spread of phytopathogens and the degradation of agroecosystems necessitate the development of innovative plant protection solutions. Traditional chemical pesticides are losing their effectiveness due to the emergence of resistant pathogens and their adverse environmental impacts, thereby intensifying interest in biological control methods. This study examines the integration of metagenomic analysis and phototrophic biotechnology as a promising approach to biocontrol. Metagenomics enables the precise identification of phytopathogens and beneficial microorganisms, laying the groundwork for the development of targeted biopesticides. Phototrophic microorganisms, including microalgae and cyanobacteria, exhibit antimicrobial properties and contribute to the restoration of soil ecosystems. The convergence of these technologies offers opportunities to form adaptive microbial consortia that ensure the long-term sustainability of agroecosystems. The paper discusses key challenges, including data processing complexities, the scalability of technologies, and regulatory barriers, and underscores the need for standardized methodologies and interdisciplinary collaboration. The integration of metagenomics and phototrophic biotechnology represents a promising direction for creating environmentally safe and sustainable agricultural production systems. © 2025 Elsevier B.V., All rights reserved.Publication Metadata only Strategies for genetic modification of microalgae to improve the production efficiency of liquid biofuel(Elsevier Ltd, 2025) Kossalbayev, Bekzhan D.; Kakimova, Ardak B.; Sadvakasova, Asemgul K.; Bauenova, Meruyert O.; Balouch, Huma; Lyaguta, Mariya A.; Ahmad, Fiaz; Kirbayeva, Dariga K.; Özgül, Sevim; Allakhverdiev, Suleyman I.; Kossalbayev, Bekzhan D., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan; Kakimova, Ardak B., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an, China; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Bauenova, Meruyert O., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Balouch, Huma, Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Lyaguta, Mariya A., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Ahmad, Fiaz, Key Laboratory for Space Bioscience and Biotechnology, Northwestern Polytechnical University, Xi'an, China; Kirbayeva, Dariga K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Özgül, Sevim, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, Turkey; Allakhverdiev, Suleyman I., Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, Turkey, Controlled Photobiosynthesis Laboratory, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian FederationThe application of genetic engineering in biofuel production has advanced significantly, driven by developments in genetic tools and omics technologies. These advancements have enhanced our understanding of lipid and carbohydrate metabolism, opening new avenues for metabolic engineering to optimize biofuel production. This review explores genetic strategies to improve the lipid content and fatty acid profiles for biodiesel production, as well as innovations in engineering for one-step biobutanol synthesis using cyanobacteria. Strategies for carbohydrate accumulation are also examined, highlighting their role in biofuel production. Additionally, the review evaluates the environmental risks associated with large-scale fourth-generation biofuel production. The findings emphasize the potential of genetic engineering to transform microalgae into highly efficient biofuel platforms capable of producing biodiesel, biobutanol, and other liquid biofuels. By addressing critical challenges and leveraging cutting-edge technologies, this research contributes to the development of sustainable and economically viable biofuel production systems. © 2025 Elsevier B.V., All rights reserved.Publication Metadata only Progress and innovation in key technologies for converting biomass to hydrogen(Elsevier Ltd, 2025) Kamshybayeva, Gulzhanay K.; Sadvakasova, Asemgul K.; Belkozhayev, Ayaz; Kossalbayev, Bekzhan D.; Bauenova, Meruyert O.; Zharmukhamedov, Sergei K.; Hou, Harvey J.M.; Allakhverdiev, Suleyman I.; Kamshybayeva, Gulzhanay K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Belkozhayev, Ayaz, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Bauenova, Meruyert O., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Zharmukhamedov, Sergei K., Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation; Hou, Harvey J.M., Department of Physical and Forensic Sciences, Alabama State University, Montgomery, United States; Allakhverdiev, Suleyman I., Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation, Controlled Photobiosynthesis Laboratory, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, TurkeyThe growing demand for clean energy has spotlighted biomass as a promising resource for sustainable hydrogen production, providing a carbon-neutral alternative to traditional fossil fuels. This review examines the latest advancements in converting biomass to hydrogen, focusing on thermochemical methods like gasification and pyrolysis, catalyst development, and biotechnological approaches such as dark fermentation and biophotolysis. While these methods offer substantial environmental benefits, including waste reduction and renewable energy generation, challenges persist in optimizing feedstock diversity, enhancing catalyst stability, and achieving cost-effective scalability. Innovations in plasma-assisted reforming, advanced nanocatalysts, and integrated reactor designs show promise in overcoming these barriers. By fostering collaboration across academia, industry, and government, these advancements can pave the way for a viable, sustainable hydrogen economy and contribute significantly to reducing global carbon emissions. © 2025 Elsevier B.V., All rights reserved.Publication Metadata only Development and Transfer of Microbial Agrobiotechnologies in Contrasting Agrosystems: Experience of Kazakhstan and China(Multidisciplinary Digital Publishing Institute (MDPI), 2025) Nygymetova, Aimeken M.; Sadvakasova, Asemgul K.; Zaletova, Dilnaz E.; Kossalbayev, Bekzhan D.; Bauenova, Meruyert O.; Wang, Jingjing; Huang, Zhiyong; Sarsekeyeva, Fariza K.; Kirbayeva, Dariga K.; Allakhverdiev, Suleyman I.; Nygymetova, Aimeken M., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Sadvakasova, Asemgul K., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Zaletova, Dilnaz E., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Tianjin Institute of Industrial Biotechnology, Tianjin, China, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan; Bauenova, Meruyert O., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Wang, Jingjing, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Huang, Zhiyong, Tianjin Institute of Industrial Biotechnology, Tianjin, China; Sarsekeyeva, Fariza K., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kirbayeva, Dariga K., Department of Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Allakhverdiev, Suleyman I., Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, Turkey, Institute of Fundamental Problems of Biology of the Russian Academy of Sciences, Pushchino, Russian Federation, Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian FederationThe development and implementation of microbial consortium-based biofertilizers represent a promising direction in sustainable agriculture, particularly in the context of the ongoing global ecological and agricultural crisis. This article examines the agroecological and economic impacts of applying microbial consortiums and explores the mechanisms of technology transfer using the example of two countries with differing levels of scientific and technological advancement–China and Kazakhstan. The analysis of the Chinese experience reveals that the successful integration of microbial biofertilizers into agricultural practice is made possible by a well-established institutional framework that includes strong governmental support for R&D, a robust scientific infrastructure, and effective coordination with the private sector. In contrast, Kazakhstan, despite its favorable agroecological conditions and growing interest among farmers in environmentally friendly technologies, faces several challenges from limited funding to a fragmented technology transfer system. The comparative study demonstrates that adapting Chinese models requires consideration of local specificities and the strengthening of intergovernmental cooperation. The article concludes by emphasizing the need to establish a multi-level innovation ecosystem encompassing the entire cycle of development and deployment of microbial biofertilizers, as a prerequisite for improving agricultural productivity and ensuring food security in countries at different stages of economic development. © 2025 Elsevier B.V., All rights reserved.Publication Metadata only In silico design of biomass-to-hydrogen Pathways: A review(Elsevier Ltd, 2025) Kakimova, Ardak B.; Sadvakasova, Asemgul K.; Kossalbayev, Bekzhan D.; Zadneprovskaya, Elena V.; Xu, Tao; Zaletova, Dilnaz E.; Allakhverdiev, Suleyman I.; Kakimova, Ardak B., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan, Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan; Sadvakasova, Asemgul K., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Kossalbayev, Bekzhan D., Department of Chemical and Biochemical Engineering, Satbayev University, Almaty, Kazakhstan, Ecology Research Institute, Khoja Akhmet Yassawi International Kazakh-Turkish University, Turkistan, Kazakhstan; Zadneprovskaya, Elena V., Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation; Xu, Tao, College of Energy Engineering, Xi'an University of Science and Technology, Xi'an, China; Zaletova, Dilnaz E., Faculty of Biology and Biotechnology, Al Farabi Kazakh National University, Almaty, Kazakhstan; Allakhverdiev, Suleyman I., Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russian Federation, Faculty of Engineering and Natural Sciences, Bahçeşehir Üniversitesi, Istanbul, TurkeyHydrogen production from biomass has emerged as a promising renewable energy solution. However, significant challenges such as thermodynamic inefficiencies, high raw material costs, low hydrogen molar yields, and difficulties in using lignocellulosic feedstocks hinder its large-scale implementation. Conventional methods have not been able to effectively address these issues, which makes modern approaches, such as in silico strategies, essential. This review explores the role of computational models like genome-scale metabolic modeling, synthetic biology, and metabolic pathway reconstruction in overcoming these barriers. By utilizing vast genomic databases and advanced computational tools, researchers can optimize microbial systems, improve hydrogen yields, and design more efficient biohydrogen production processes. These in silico methods provide a pathway to enhance the efficiency of biomass processing and enable the development of scalable and sustainable hydrogen production technologies. The review highlights recent advancements and discusses the potential of in silico approaches to address key technological and economic limitations, paving the way for the future of biohydrogen energy. © 2025 Elsevier B.V., All rights reserved.