Green-Synthesized Silver Nanoparticles and the Effect on Human Neuroblastoma Cells

Abstract

Neuroblastoma is a complex disease that shares some cellular traits with neurons involved in neurodegenerative diseases like Alzheimer's and Parkinson's diseased. The human neuroblastoma SH-SY5Y cell line is commonly used to model these conditions, providing a useful platform for exploring potential treatments. Silver nanoparticles (AgNPs) have gained attention as promising candidates due to their distinctive properties, although there are ongoing concerns regarding the toxicity and environmental effects linked to traditional synthesis methods. This study investigated the green synthesis of AgNPs using Aloe vera extract and assessed their effects on the viability of human neuroblastoma SH-SY5Y cells. Various techniques were employed to characterize the synthesized AgNPs, confirming their successful formation and stability. The MTT assay demonstrated a dose-dependent cytotoxic effect of AgNPs on SH-SY5Y cells, with lower concentrations showing acceptable biocompatibility. This research highlights the potential of green synthesized AgNPs as a viable therapeutic option for neuroblastoma, while also emphasizing the importance of careful concentration management. Moreover, this study aims to add to this expanding field by concentrating on the green synthesis of AgNPs using Aloe vera and assessing their therapeutic potential in relation to neurodegenerative diseases. © 2024 Elsevier B.V., All rights reserved.