Publication:
The Effect of Probiotic and Omega-3 Supplements on Total Oxidant and Total Antioxidant Levels in Experimental Colitis

Simple item page
dc.contributor.authorGünal, Mehmet Yalçın
dc.contributor.authorKızıltan, Gül
dc.contributor.authorÖzdemir, Ekrem Musa
dc.contributor.authorKılıç, Ülkan
dc.contributor.authorOzansoy, Mehmet
dc.contributor.authorKeskin, İlknur
dc.contributor.authorYoldaş İlktaç, Havvanur
dc.contributor.authorÖzmen Togay, Sine
dc.contributor.institutionALANYA ALAADDİN KEYKUBAT ÜNİVERSİTESİ
dc.contributor.institutionBAŞKENT ÜNİVERSİTESİ
dc.contributor.institutionİSTANBUL MEDİPOL ÜNİVERSİTESİ
dc.contributor.institutionSAĞLIK BİLİMLERİ ÜNİVERSİTESİ
dc.contributor.institutionBAHÇEŞEHİR ÜNİVERSİTESİ
dc.contributor.institutionİSTANBUL MEDİPOL ÜNİVERSİTESİ
dc.contributor.institutionİSTANBUL MEDENİYET ÜNİVERSİTESİ
dc.contributor.institutionBURSA ULUDAĞ ÜNİVERSİTESİ
dc.date.accessioned2025-09-20T19:57:52Z
dc.date.issued2021
dc.date.submitted14.01.2022
dc.description.abstractObjective: Treatment of inflammatory bowel disease (IBD) usually involves medical therapy. For this reason, it is recommended to seek alternative treatment methods such as nutritional therapy. The aim of the study is to evaluate the effects of probiotics and omega-3 fatty acids on total oxidant and total antioxidant levels in an experimental colitis model. Methods: Mice were randomly divided into five groups (n= 10/group) as healthy group, colitis group, group treated with probiotics (VSL#3), group treated with omega-3 (w-3), and group treated with both probiotics and omega-3. To induce experimental colitis, 200 mg/kg dinitrobenzene sulfonic acid (DNBS) + 30% ethanol combination was rectally administered to anesthetized mice. Total oxidant (TOS) and total antioxidant (TAS) levels were measured at the tissue level. Results: Lower concentrations of TOS were observed in the probiotics groups (2.11±0.23 mmol H2O2 Eq/L), probiotics+omega-3 (2.56±1.18 mmol H2O2 Eq/L), and omega-3 (3.02±1.88 mmol H2O2 Eq/L) groups compared to the colitis group (3.11±0.91 mmol H2O2 Eq/L) (p>0.05). Higher TOS and TAS level were observed in the control colitis group compared to other groups, however, the differences were not statistically significant. Conclusion: Our findings showed that TAS and TOS levels were positively affected by the use of probiotic supplements in IBD. It was determined that using w-3 alone was ineffective in decreasing TOS levels. Studies with higher dosages and longer treatment periods are needed to better observe the effects of nutritional supplements on TOS and TAS parameters in inflammatory bowel diseases.
dc.identifier.doi10.33808/clinexphealthsci.865058
dc.identifier.endpage366
dc.identifier.issn2459-1459
dc.identifier.issue2
dc.identifier.startpage362
dc.identifier.urihttps://hdl.handle.net/20.500.14719/4904
dc.identifier.volume11
dc.language.isoen
dc.relation.journalClinical and Experimental Health Sciences
dc.subjectBiyoloji
dc.subjectGenel ve Dahili Tıp
dc.titleThe Effect of Probiotic and Omega-3 Supplements on Total Oxidant and Total Antioxidant Levels in Experimental Colitis
dc.typeResearch Article
dcterms.references[1] Baumgart DC, Carding SR. Inflammatory bowel disease: cause and immunobiology. Lancet 2007, 369(9573): 1627–1640. doi: 10.1016/S0140-6736(07)60750-8,[2] Sands BE. From symptom to diagnosis: clinical distinctions among various forms of intestinal inflammation. Gastroenterology 2004, 126(6): 1518–32. doi: 10.1053/j. gastro.2004.02.072,[3] Sarma A, Rahaman Mallick A, Ghosh A. Free radicals and their role in different clinical conditions: an overview. Int J Pharma Sci Res. 2010, 1(3): 185-192.,[4] Satsangi J, Silverberg M, Vermeire S, Colombel J-F. Satsangi J, Silverberg MS, Vermeire S, Colombel JF. The Montreal classification of inflammatory bowel disease: controversies, consensus, and implications. Gut 2006, 55(6): 749-753. doi: 10.1136/gut.2005.082909,[5] Buyukuslu N, Yigitbasi T. Reactive oxygen species and oxidative stress in obesity. MÜSBED 2015, 5(3): 197-203. doi: 10.5455/ musbed.201.506.04061607 (Turkish),[6] Aghdassi E, Wendland BE, Steinhart AH, Wolman SL, Jeejeebhoy K, Allard JP. Antioxidant vitamin supplementation in Crohn’s disease decreases oxidative stress. a randomized controlled trial. Am J Gastroenterol. 2003, 98(2): 348–53. doi: 10.1111/j.1572-0241.2003.07226.x,[7] Catarzi S, Favilli F, Romagnoli C, Marcucci T, Picariello L, Tonelli F, Vincenzini MT, Iantomasi T. Oxidative state and IL-6 production in intestinal myofibroblasts of Crohn’s disease patients. Inflamm Bowel Dis. 2011, 17(8): 1674–1684. doi: 10.1002/ibd.21552,[8] Keshavarzian A, Banan A, Farhadi A, Komanduri S, Mutlu E, Zhang Y, Fields ZJ. Increases in free radicals and cytoskeletal protein oxidation and nitration in the colon of patients with inflammatory bowel disease. Gut 2003, 52(5): 720–728.,[9] Krzystek-Korpacka M, Neubauer K, Berdowska I, Zielinski B, Paradowski L, Gamian A. Impaired erythrocyte antioxidant defense in active inflammatory bowel disease: impact of anemia and treatment. Inflamm Bowel Dis. 2010, 16(9): 1467– 1475. doi: 10.1002/ibd.21234,[10] Pinto MAS, Lopes MS-MS, Bastos STO, Reigada CLL, Dantas RF, Neto JCB, Luna SA, Madi K, Nunes T, Zaltman C. Does active Crohn’s disease have decreased intestinal antioxidant capacity? J Crohns Colitis. 2013, 7(9): e358-366. doi: 10.1016/j. crohns.2013.02.010,[11] Breganó JW, Dichi JB, Barbosa DS, El Kadri MZ, Matsuo T, Rodrigues MA, Cecchini R, Dichi I. Decreased total antioxidant capacity in plasma, but not tissue, in experimental colitis. Dig Dis Sci. 2009, 54(4): 751–757. doi: 10.1007/s10620.008.0439-z,[12] Kruidenier L, Kuiper I, Van Duijn W, Mieremet-Ooms MAC, van Hogezand RA, Lamers CBHW, Verspaget HW. Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. J Pathol. 2003, 201(1): 17–27. doi: 10.1002/ path.1408,[13] Kruidenier L, Kuiper I, Lamers CBHW, Verspaget HW. Intestinal oxidative damage in inflammatory bowel disease: semiquantification, localization, and association with mucosal antioxidants. J Pathol. 2003, 201(1): 28–36. doi: 10.1002/ path.1409,[14] Özgün E, Özgün GS, Eskiocak S, Yalçin Ö, Gökmen SS. Deneysel kolitte L-karnitinin serum paraoksonaz, arilesteraz ve laktonaz aktivitelerine ve oksidatif duruma etkisi. Turkish J Biochem. 2013, 38(2): 145–153. doi: 10.5505/tjb.2013.29292 (Turkish),[15] Del Rio D, Stewart AJ, Pellegrini N. A review of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr Metab Cardiovasc Dis. 2005, 15(4): 316–328. doi: 10.1016/j.numecd.2005.05.003,[16] Erel O. A new automated colorimetric method for measuring total oxidant status. Clin Biochem. 2005, 38(12): 1103–1111. doi: 10.1016/j.clinbiochem.2005.08.008,[17] Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004, 37(4): 277–285. doi: 10.1016/j.clinbiochem.2003.11.015,[18] Cosnes J, Gower-Rousseau C, Seksik P, Cortot A. Epidemiology and natural history of inflammatory bowel diseases. Gastroenterology 2011, 140(6): 1785–94. doi: 10.1053/j. gastro.2011.01.055,[19] Biancone L, Calabrese E, Petruzziello C, Pallone F. Treatment with biologic therapies and the risk of cancer in patients with IBD. Nat Clin Pract Gastroenterol Hepatol. 2007, 4(2): 78–91. doi: 10.1038/ncpgasthep0695,[20] Calder PC. The relationship between the fatty acid composition of immune cells and their function. Prostaglandins Leukot Essent Fatty Acids. 2008, 79(3–5): 101–108. doi: 10.1016/j. plefa.2008.09.016,[21] Bassaganya-Riera J, Viladomiu M, Pedragosa M, De Simone C, Carbo A, Shaykhutdinov R, Jobin C, Arthur JC, Corl BA, Vogel H, Storr M, Hontecillas R. Probiotic bacteria produce conjugated linoleic acid locally in the gut that targets macrophage PPAR γ to suppress colitis. PLoS One 2012,7(2):e31238. doi: 10.1371/ journal.pone.0031238,[22] Ersan LY, Topçuoğlu E. Microbiological and some physico-chemical properties of probiotic yogurt enriched with almond milk. Bursa Uludag Üniv Ziraat Fak Derg. 2019, 33(2): 321–339. (Turkish),[23] Martín R, Chain F, Miquel S, Lu J, Gratadoux J-J, Sokol H, Verdu EF, Bercik P, Bermudez-Humaran LG, Langella P. The commensal bacterium Faecalibacterium prausnitzii is protective in DNBS-induced chronic moderate and severe colitis models. Inflamm Bowel Dis. 2014, 20(3): 417–430. doi: 10.1097/01. MIB.000.044.0815.76627.64,[24] Iborra M, Moret I, Rausell F, Bastida G, Aguas M, Cerrillo E, Nos P, Beltran B. Role of oxidative stress and antioxidant enzymes in Crohn’s disease. Biochem Soc Trans. 2011, 39(4): 1102–6. doi: 10.1042/BST0391102,[25] Yuksel MD, Ates I, Kaplan M, Arikan M, Ozin Y, Mesut Z, Topcuoglu C, Kayacetimn E. Is oxidative stress associated with activation and pathogenesis of inflammatory bowel disease? J Med Biochem. 2017, 36(4): 341–348. doi: 10.1515/jomb-2017-0013,[26] Matsuda H, Fujiyama Y, Andoh A, Ushijima T, Kajinami T, Bamba T. Characterization of antibody responses against rectal mucosa-associated bacterial flora in patients with ulcerative colitis. J Gastroenterol Hepatol. 2000, 15(1): 61–68. doi: 10.1046/j.1440-1746.2000.02045.x,[27] Bitiren M, Karakilcik AZ, Zerin M, Ozardali I, Selek S, Nazligül Y, Ozgonul A, Musa D, Uzunkoy A. Protective effects of selenium and vitamin E combination on experimental colitis in blood plasma and colon of rats. Biol Trace Elem Res. 2010, 136(1): 87–95. doi: 10.1007/s12011.009.8518-3,[28] Özkan N. Sıçanlarda trinitrobenzene sulphonic acid (TNBS) ile uyarılan kolit modelinde kefirin koruyucu etkinliğinin araştırılması. Süleyman Demirel Üniversitesi Tıp Fakültesi İç Hastalıkları Anabilim Dalı Uzmanlık Tezi, Isparta, 2008. (Turkish),[29] Osman N, Adawi D, Molin G, Ahrne S, Berggren A, Jeppsson B. Bifidobacterium infantis strains with and without a combination of oligofructose and inulin (OFI) attenuate inflammation in DSS-induced colitis in rats. BMC Gastroenterol. 2006, 6: 31. doi: doi: 10.1186/1471-230X-6-31,[30] Mañé J, Lorén V, Pedrosa E, Ojanguren I, Xaus J, Cabré E, Domenech E, Gassul MA. Lactobacillus fermentum CECT 5716 prevents and reverts intestinal damage on TNBS-induced colitis in mice. Inflamm Bowel Dis. 2009, 15(8): 1155–1163. doi: 10.1002/ibd.20908,[31] Mazloom Z, Yousefinejad A, Dabbaghmanesh MH. Effect of probiotics on lipid profile, glycemic control, insulin action, oxidative stress, and inflammatory markers in patients with type 2 diabetes: a clinical trial. Iran J Med Sci. 2013, 38(1): 38–43.,[32] Lamprecht M, Bogner S, Schippinger G, Steinbauer K, Fankhauser F, Hallstroem S, Schuetz B, Greilberger JF. Probiotic supplementation affects markers of intestinal barrier, oxidation, and inflammation in trained men, a randomized, double-blinded, placebo-controlled trial. J Int Soc Sports Nutr. 2012, 9(1): 45. doi: 10.1186/1550-2783-9-45,[33] Turner D, Zlotkin SH, Shah PS, Griffiths AM. Omega 3 fatty acids (fish oil) for maintenance of remission in Crohn’s disease. Cochrane database Syst Rev. 2007, (2): CD006320. doi: 10.1002/14651858.CD006320.pub2,[34] Morampudi V, Bhinder G, Wu X, Dai C, Sham HP, Vallance BA, Jacobson K. DNBS/TNBS colitis models: providing insights into inflammatory bowel disease and effects of dietary fat. J Vis Exp. 2014, 84: e51297–e51297. doi: 10.3791/51297,[35] Nieto N, Torres MI, Fernández MI, Girón MD, Ríos A, Suárez MD, Gil A. Experimental ulcerative colitis impairs antioxidant defense system in rat intestine. Dig Dis Sci. 2000, 45(9): 1820– 1827. doi: 10.1023/a:100.556.5708038,[36] Maor I, Rainis T, Lanir A, Lavy A. Oxidative stress, inflammation and neutrophil superoxide release in patients with Crohn’s disease: distinction between active and non-active disease. Dig Dis Sci. 2008, 53(8): 2208–2214. doi: 10.1007/ s10620.007.0141-6,[37] Koutroubakis I, Malliaraki N, Dimoulios P, Karmiris K, Castanas E, Kouroumalis E. Decreased total and corrected antioxidant capacity in patients with inflammatory bowel disease. Dig Dis Sci. 2004, 49: 1433–1437. doi: 10.1023/B:DDAS.000.004.2242.22898.d9,[38] D’Odorico A, Bortolan S, Cardin R, D’Inca’ R, Martines D, Ferronato A, Sturniola GC. Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease. Scand J Gastroenterol. 2001, 36(12): 1289–1294. doi: 10.1080/003.655.201317097146,[39] Peran L, Camuesco D, Comalada M, Nieto A, Concha A, DiazRopero MP, Olivares M, Xaus J, Zarzuelo A, Galvez J. Preventative effects of a probiotic, Lactobacillus salivarius ssp. salivarius, in the TNBS model of rat colitis. World J Gastroenterol. 2005, 11(33): 5185–5192. doi: 10.3748/wjg.v11.i33.5185,[40] Peran L, Camuesco D, Comalada M, Bailon E, Henriksson A, Xaus J, Galvez J. A comparative study of the preventative effects exerted by three probiotics, Bifidobacterium lactis, Lactobacillus casei and Lactobacillus acidophilus, in the TNBS model of rat colitis. J Appl Microbiol. 2007, 103(4): 836–844. doi: 10.1111/j.1365-2672.2007.03302.x,[41] Turner D, Shah PS, Steinhart AH, Zlotkin S, Griffiths AM. Maintenance of remission in inflammatory bowel disease using omega-3 fatty acids (fish oil): a systematic review and meta-analyses. Inflamm Bowel Dis. 2011, 17(1): 336–345. doi: 10.1002/ibd.21374,[42] Lev-Tzion R, Griffiths AM, Leder O, Turner D. Omega 3 fatty acids (fish oil) for maintenance of remission in Crohn’s disease. Cochrane database Syst Rev. 2014, 2: CD006320. doi: 10.1002/14651858.CD006320.pub4,[43] Barbosa DS, Cecchini R, El Kadri MZ, Rodríguez MAM, Burini RC, Dichi I. Decreased oxidative stress in patients with ulcerative colitis supplemented with fish oil omega-3 fatty acids. Nutrition 2003, 19(10): 837–842. doi: 10.1016/s0899- 9007(03)00162-x
dspace.entity.typePublication
local.indexed.atTRDizin

Files

Original bundle

Now showing 1 - 1 of 1
No Thumbnail Available
Name:
The Effect of Probiotic and Omega-3 Supplements on Total Oxidant and Total Antioxidant Levels in Experimental Colitis.pdf
Size:
311.18 KB
Format:
Adobe Portable Document Format
Download

Collections

TR-Dizin İndeksli Yayınlar Koleksiyonu