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Development of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning

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dc.contributor.authorYalçın Özkumur, Ayça
dc.contributor.authorGoods, Brittany A.
dc.contributor.authorLove, J. Christopher
dc.contributor.institutionYalcin Ozkumur, Ayca, Department of Electrical and Electronic Engineering, Bahçeşehir Üniversitesi, Istanbul, Turkey
dc.contributor.institutionGoods, Brittany A., Massachusetts Institute of Technology, Cambridge, United States
dc.contributor.institutionLove, J. Christopher, Massachusetts Institute of Technology, Cambridge, United States, Broad Institute, Cambridge, United States, Ragon Institute of MGH, Massachusetts Institute of Technology, Cambridge, United States
dc.date.accessioned2025-10-05T16:30:34Z
dc.date.issued2015
dc.description.abstractLiving-cell-based screens can facilitate lead discovery of functional therapeutics of interest. A versatile and scalable method is reported that uses dense arrays of nanowells for imparting defined patterns on monolayers of cells. It is shown that this approach can coordinate a multi-component biological assay by designing and implementing a high-throughput, functional nanoliter-scale neutralization assay to identify neutralizing antibodies against HIV. © 2015 Elsevier B.V., All rights reserved.
dc.identifier.doi10.1002/smll.201500674
dc.identifier.endpage4650
dc.identifier.issn16136829
dc.identifier.issn16136810
dc.identifier.issue36
dc.identifier.pubmed26121321
dc.identifier.scopus2-s2.0-84942369514
dc.identifier.startpage4643
dc.identifier.urihttps://doi.org/10.1002/smll.201500674
dc.identifier.urihttps://hdl.handle.net/20.500.14719/12707
dc.identifier.volume11
dc.language.isoen
dc.publisherWiley-VCH Verlag info@wiley-vch.de
dc.relation.oastatusAll Open Access
dc.relation.oastatusBronze Open Access
dc.relation.oastatusGreen Accepted Open Access
dc.relation.oastatusGreen Final Open Access
dc.relation.oastatusGreen Open Access
dc.relation.sourceSmall
dc.subject.authorkeywordsCell Patterning
dc.subject.authorkeywordsHuman Immunodeficiency Virus (hiv)
dc.subject.authorkeywordsLiving-cell Arrays
dc.subject.authorkeywordsNeutralization
dc.subject.authorkeywordsAnti-hiv Agents
dc.subject.authorkeywordsAntibodies, Neutralizing
dc.subject.authorkeywordsBiological Products
dc.subject.authorkeywordsHiv Antibodies
dc.subject.authorkeywordsCells
dc.subject.authorkeywordsCytology
dc.subject.authorkeywordsScalability
dc.subject.authorkeywordsThroughput
dc.subject.authorkeywordsViruses
dc.subject.authorkeywordsBiological Assays
dc.subject.authorkeywordsCell-patterning
dc.subject.authorkeywordsHuman Immunodeficiency Virus
dc.subject.authorkeywordsLiving Cell Array
dc.subject.authorkeywordsNanoliter Scale
dc.subject.authorkeywordsNeutralization
dc.subject.authorkeywordsNeutralizing Antibodies
dc.subject.authorkeywordsScalable Methods
dc.subject.authorkeywordsDiseases
dc.subject.authorkeywordsAnti Human Immunodeficiency Virus Agent
dc.subject.authorkeywordsBiological Product
dc.subject.authorkeywordsHuman Immunodeficiency Virus Antibody
dc.subject.authorkeywordsNanoparticle
dc.subject.authorkeywordsNeutralizing Antibody
dc.subject.authorkeywordsAnimal
dc.subject.authorkeywordsCell Motion
dc.subject.authorkeywordsCell Proliferation
dc.subject.authorkeywordsChemistry
dc.subject.authorkeywordsCho Cell Line
dc.subject.authorkeywordsCricetulus
dc.subject.authorkeywordsDrug Design
dc.subject.authorkeywordsFalse Positive Result
dc.subject.authorkeywordsFluorescence Microscopy
dc.subject.authorkeywordsHamster
dc.subject.authorkeywordsHigh Throughput Screening
dc.subject.authorkeywordsHiv Infections
dc.subject.authorkeywordsHuman
dc.subject.authorkeywordsHuman Immunodeficiency Virus 1
dc.subject.authorkeywordsImmunology
dc.subject.authorkeywordsMicrofluidics
dc.subject.authorkeywordsNanotechnology
dc.subject.authorkeywordsProcedures
dc.subject.authorkeywordsReceiver Operating Characteristic
dc.subject.authorkeywordsSerodiagnosis
dc.subject.authorkeywordsAnimals
dc.subject.authorkeywordsAnti-hiv Agents
dc.subject.authorkeywordsAntibodies, Neutralizing
dc.subject.authorkeywordsBiological Products
dc.subject.authorkeywordsCell Movement
dc.subject.authorkeywordsCell Proliferation
dc.subject.authorkeywordsCho Cells
dc.subject.authorkeywordsCricetinae
dc.subject.authorkeywordsDrug Design
dc.subject.authorkeywordsFalse Positive Reactions
dc.subject.authorkeywordsHigh-throughput Screening Assays
dc.subject.authorkeywordsHiv Antibodies
dc.subject.authorkeywordsHiv-1
dc.subject.authorkeywordsHumans
dc.subject.authorkeywordsMicrofluidics
dc.subject.authorkeywordsMicroscopy, Fluorescence
dc.subject.authorkeywordsNanoparticles
dc.subject.authorkeywordsNanotechnology
dc.subject.authorkeywordsNeutralization Tests
dc.subject.authorkeywordsRoc Curve
dc.subject.indexkeywordsCells
dc.subject.indexkeywordsCytology
dc.subject.indexkeywordsScalability
dc.subject.indexkeywordsThroughput
dc.subject.indexkeywordsViruses
dc.subject.indexkeywordsBiological assays
dc.subject.indexkeywordsCell-patterning
dc.subject.indexkeywordsHuman immunodeficiency virus
dc.subject.indexkeywordsLiving cell array
dc.subject.indexkeywordsNanoliter scale
dc.subject.indexkeywordsneutralization
dc.subject.indexkeywordsNeutralizing antibodies
dc.subject.indexkeywordsScalable methods
dc.subject.indexkeywordsDiseases
dc.subject.indexkeywordsanti human immunodeficiency virus agent
dc.subject.indexkeywordsbiological product
dc.subject.indexkeywordsHuman immunodeficiency virus antibody
dc.subject.indexkeywordsnanoparticle
dc.subject.indexkeywordsneutralizing antibody
dc.subject.indexkeywordsanimal
dc.subject.indexkeywordscell motion
dc.subject.indexkeywordscell proliferation
dc.subject.indexkeywordschemistry
dc.subject.indexkeywordsCHO cell line
dc.subject.indexkeywordsCricetulus
dc.subject.indexkeywordsdrug design
dc.subject.indexkeywordsfalse positive result
dc.subject.indexkeywordsfluorescence microscopy
dc.subject.indexkeywordshamster
dc.subject.indexkeywordshigh throughput screening
dc.subject.indexkeywordsHIV Infections
dc.subject.indexkeywordshuman
dc.subject.indexkeywordsHuman immunodeficiency virus 1
dc.subject.indexkeywordsimmunology
dc.subject.indexkeywordsmicrofluidics
dc.subject.indexkeywordsnanotechnology
dc.subject.indexkeywordsprocedures
dc.subject.indexkeywordsreceiver operating characteristic
dc.subject.indexkeywordsserodiagnosis
dc.subject.indexkeywordsAnimals
dc.subject.indexkeywordsAnti-HIV Agents
dc.subject.indexkeywordsAntibodies, Neutralizing
dc.subject.indexkeywordsBiological Products
dc.subject.indexkeywordsCell Movement
dc.subject.indexkeywordsCell Proliferation
dc.subject.indexkeywordsCHO Cells
dc.subject.indexkeywordsCricetinae
dc.subject.indexkeywordsDrug Design
dc.subject.indexkeywordsFalse Positive Reactions
dc.subject.indexkeywordsHigh-Throughput Screening Assays
dc.subject.indexkeywordsHIV Antibodies
dc.subject.indexkeywordsHIV-1
dc.subject.indexkeywordsHumans
dc.subject.indexkeywordsMicrofluidics
dc.subject.indexkeywordsMicroscopy, Fluorescence
dc.subject.indexkeywordsNanoparticles
dc.subject.indexkeywordsNanotechnology
dc.subject.indexkeywordsNeutralization Tests
dc.subject.indexkeywordsROC Curve
dc.titleDevelopment of a High-Throughput Functional Screen Using Nanowell-Assisted Cell Patterning
dc.typeArticle
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local.indexed.atScopus
person.identifier.scopus-author-id11640910300
person.identifier.scopus-author-id56652211200
person.identifier.scopus-author-id7202207769

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